Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 9.0, 0.2 micron filtered
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
| CAS | 1135-40-6 |
|---|---|
| Molecular Weight (g/mol) | 221.32 |
| MDL Number | MFCD00003837 |
| Physical Form | Liquid |
| pH | 9.0 |
| SMILES | OS(=O)(=O)CCCNC1CCCCC1 |
| Recommended Storage | Ambient temperatures |
| Concentration | 0.5 M |
| IUPAC Name | 3-(cyclohexylamino)propane-1-sulfonic acid |
| InChI Key | PJWWRFATQTVXHA-UHFFFAOYSA-N |
| Molecular Formula | C9H19NO3S |
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 8.8
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Gibco™ HBSS (10X), calcium, magnesium, phenol red
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Includes | Calcium and Magnesium |
|---|---|
| Physical Form | Liquid |
| pH | 5.5 to 6.0 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Hanks' Balanced Salt Solution (HBSS) |
| Recommended Storage | Storage conditions: 15°C to 30°C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
| Concentration | 10X |
| Osmolality | 2500 to 3000 mOsm/kg |
| Sterility | Sterile-filtered |
| Solution Type | Hank's Balanced Salt |
| Product Line | Gibco |
| Name Note | low endotoxin |
|---|---|
| Storage Note 1 | 2° to 8°C |
| Color | Colorless |
| Physical Form | Liquid |
| Chemical Name or Material | BES-buffered saline (2X) |
| TSCA | No |
| Recommended Storage | Keep cold |
MP Biomedicals™ 1X Hanks' Balanced Salt Solution (Modified) without Magnesium and Calcium
Minimize cell attachment or aggregation.
Invitrogen™ 10X TBE (powder)
Invitrogen 10X TBE (Tris-Borate-EDTA Buffer) is thoroughly tested for RNase and DNase contamination, making it ideal for use in sensitive application.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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N,N-Di(2-hydroxyethyl)glycine 99.0+%, TCI America™
CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: sodium 2-[bis(2-hydroxyethyl)amino]acetate SMILES: [Na+].OCCN(CCO)CC([O-])=O
| PubChem CID | 8761 |
|---|---|
| CAS | 150-25-4 |
| Molecular Weight (g/mol) | 185.16 |
| ChEBI | CHEBI:40957 |
| MDL Number | MFCD00004295 |
| SMILES | [Na+].OCCN(CCO)CC([O-])=O |
| Synonym | bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine |
| IUPAC Name | sodium 2-[bis(2-hydroxyethyl)amino]acetate |
| InChI Key | MFBDBXAVPLFMNJ-UHFFFAOYSA-M |
| Molecular Formula | C6H12NNaO4 |
StatLab Poly Scientific Phosphate Buffered Saline 0.1M pH 7.2
For preserving tissues during surgical procedures and IHC staining procedures.
Buffer Solution, for Nitrate Determination using Ion Selective Electrode, Ricca Chemical
Nitrate Determination using Ion Selective Electrode
| Color | Colorless |
|---|---|
| Packaging | Amber Glass Bottle |
| Physical Form | Liquid |
| pH | 2.98 to 3.98 |
| Chemical Name or Material | Buffer Solution |
| Grade | Chemical Solution |
| For Use With (Application) | Ion Selective Analysis |
| Solution Type | Buffer |
| Melting Point | ∼ 0°C |
Buffer Solution A, for Sulfate Analysis (Turbidimetric Method), Ricca Chemical
For Analysis of Sulfate (Turbidimetric Method)
| Color | Colorless |
|---|---|
| Packaging | Natural Poly Bottle |
| Physical Form | Liquid |
| Chemical Name or Material | Buffer Solution |
| Grade | Chemical Solution |
| For Use With (Application) | Sulfate Analysis (Turbidimetric Method) |
| Solution Type | Buffer |
| Melting Point | ∼ 0°C |
| Percent Purity | 99.8% to 100.2% |
|---|---|
| CAS | 77-86-1 |
| For Use With (Equipment) | Instruments and Electrodes |
| Molecular Weight (g/mol) | 121.135 |
| Physical Form | Crystalline Powder |
| pH | 10.0 to 11.5 for 5% solution |
| Grade | ACS |
| For Use With (Application) | Laboratory |
| Solution Type | Standard |
Tris(hydroxymethyl)aminomethane, ACS Reagent (Tris; THAM; 2-Amino-2-(hydroxymethyl)-1,3-propanediol), Reagents
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.14 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tromethamine IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: NC(CO)(CO)CO
| CAS | 77-86-1 |
|---|---|
| Molecular Weight (g/mol) | 121.14 |
| SMILES | NC(CO)(CO)CO |
| Synonym | Tromethamine |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |